EasyGo!™ Mouse TNF-α One-Step ELISA Kit Protein Markers

EasyGo!™ Mouse TNF-α One-Step ELISA Kit Protein MarkersSpecifications Name EasyGo! Mouse TNF One Step ELISA Kit Hgnc ID 11892 Specie mouse Assay Type sandwich Assay Time 1. 5h Sample Type cell culture supernatant Sample Amount cell culture supernatant: 100 L Sensitivity 1. 37 pg mL Range Standard 10. 94 700 pg ml Recovery 75% 128% Average Recovery 97% Intra Precision 2. 3% 6. 5% Inter Precision 5. 3% 9. 2% Plate Type Detachable 96 well Plate Components 1 Washing Buffer 1 Assay Buffer Stop Solution

and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome

characterized by the lack of the A subunit alone

A sheep polyclonal anti-trypsin capture antibody is coated on microtiter strips

10X SpeedyOne buffer Recognition Site 5'…-A↓CTAGT-…3' 3'…-TGATC↑A-…5' Reaction Buffer 10X SpeedyOne Buffer Recommended Reaction Temperature 37°C Unit Definition At 37°C

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using P290203(CARS1 Antibody) at dilution 1/50

It phosphorylates and thus activates MAPK14/p38-MAPK

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue or LCN2 antigen-treated (Neutralization experiment) using P102430(LCN2 Antibody) at dilution 1/50

8% Plate Type Detachable 96-well plate Components

Assay Procedure

P291377-WB-1-P

Equipped with suction-cup feet for high-speed stability and minimal vibration

42 pg/mL Range Standard 125 pg/mL – 8000 pg/mL Recovery 84% – 107% Average Recovery 98% Intra Precision 2